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Characterization of innately decellularised micropattern pseudostem of Musa balbisiana - A non-surface functionalized 3D economic biomaterial scaffold

Deepa Narayanan*, Sarita G Bhat & Gaurav Baranwal

Deepa Narayanan*

Department of Biotechnology, Cochin University of Science and Technology, Ernakulum-682022, Kerala, India

Sarita G Bhat

Department of Biotechnology, Cochin University of Science and Technology, Ernakulum-682022, Kerala, India

Gaurav Baranwal

Department of Medicla Physiology, College of Medicine, Texas A & M University, 2403(C) Bryan, TX 77807, USA


30 September 2021


Banana (Musa balbisiana) pseudostem 3D scaffolds have been developed here for primary eukaryotic cell and cell line culture as an economical, sustainable, eco-friendly alternative for surface-functionalized polymeric and plant tissue-based structures. Musa pseudostem 3D micro pattern scaffold (MPM-3Ds) developed by freeze-drying followed by ethylene oxide sterilization yielded 5.6ng of DNA per mg of tissue, confirming its extended decellularised state. Thermogravimetric analysis, contact angle measurement, uniaxial testing, and FTIR determined thermal stability, wettability, tensile strength, and surface functional groups respectively. Micro and macronutrients, sugars, and amino acids that naturally enrich MPM-3Ds were estimated using EDAX, HPLC, and biochemical analysis. The most important finding was, non-surface functionalized MPM-3Ds supported attachment, growth, and differentiation of human mesenchyme stem cells, human primary hepatocytes like cells, primary mouse brain cortical neurons, mouse fibroblast cells, and human pancreatic cancer cells. MPM-3Ds showed in vivo biodegradation and biocompatibility in a preliminary analysis in Sprague Dawley rats. These findings illuminate nature's power to nurture cells in the micropattern cradles of MPM- 3Ds that can support innovative research in stem cell differentiation, drug and cosmetic testing, and biosensor development leading to advanced biomedical research.


3D micropattern scaffold; banana pseudostem scaffold; economical 3D scaffold; hepatocyte cell spheroids; natural cellulosic 3D scaffold

Cite this article

Narayanan D, Bhat SG, Baranwal G (2021). Characterization of innately decellularised micropattern pseudostem of Musa balbisiana - A non-surface functionalized 3D economic biomaterial scaffold. T. Appl. Biol. Chem. J; 2(3):76-88.

Citation: 0


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Copyright: © 2021 Narayanan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.