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Influence of lead acetate on seed germination and growth of young alfalfa plants

Snežana Andjelkovič*, Snežana Babić, Jasmina Milenković, Vladimir Zornić, Miladen Prijović, Filip Bekčić and Olivera Papovič

Snežana Andjelkovič*

Institute for Forage Crops, Kruševac-37251, Serbia

https://orcid.org/0000-0003-0436-3530

Snežana Babić

Institute for Forage Crops, Kruševac-37251, Serbia

Jasmina Milenković

Institute for Forage Crops, Kruševac-37251, Serbia

Vladimir Zornić

Institute for Forage Crops, Kruševac-37251, Serbia

Miladen Prijović

Institute for Forage Crops, Kruševac-37251, Serbia

Filip Bekčić

Institute for Forage Crops, Kruševac-37251, Serbia

Olivera Papovič

Faculty of Sciences and Mathematics, University of Priština, Kosovska, Mitrovica, Serbia

Published

11 January 2022

Abstract

This study presents the results of testing the effect of different concentrations of lead-acetate on seed germination and young Seedlings of alfalfa. Тhe experiment was organized so that in the first variant, only lead-acetate of different concentrations (10-5, 10-4, 10-3, 10-2 and 2 x 10-2 M) was used, and in the second, next to the lead acetate EDTA concentration of 0.012 % was added. The results of this study in which natural conditions are imitated showed that the percentage of alfalfa seed germination decreases with increasing lead-acetate concentrations. In a medium of high concentration of lead acetate 10-2 and 2 x 10-2, a small number of seeds (39% and 32 (32%) germinated. In the same treatments with EDTA, the toxic effects of lead acetate were reduced, and seed germination was better (59% and 43% seeds germinated). It has been noticed that lead has toxic effects on the growth of alfalfa roots and stems. In the variant in which EDTA was used, the negative influence of lead on and growth of alfalfa seedlings was significantly mitigated.

Keywords

alfalfa; lead acetate; seeds; seed germination

Cite this article

Andjelkovič S, Babić S, Milenković J, Zornić V, Prijović M, Filip Bekčić, Olivera Papovič (2021). Influence of lead acetate on seed germination and growth of young alfalfa plants. T Appl. Biol. Chem. J; 2(4):89-92. https://doi.org/10.52679/tabcj.2021.0014

References

[1] Peng K, Li X, Luo C, Shen Z (2006). Vegetation composition and heavy metal uptake by wild plants at three contaminated sites in Xiangxi Area, China. J Environ Sci Health-A: Toxic/Hazard Subs Environ Eng; 40: 65–76. [CrossRef] [PubMed]

[2] Juson AEDS, Martinez MKM, Ching AJ (2016). Accumulation and distribution of heavy metals in Leucaena leucocephala Lam. and Bougainvillea spectabilis Willd. plant systems. J Exp Biol Agri Sci; 4(1): 01-06. [CrossRef]

[3] Chojnackaa K, Chojnackib A, Goreckab H, Gorecki H (2005). Bioavailability of heavy metals from polluted soils to plants. Sci Tot Environ; 337: 175–82. [CrossRef] [PubMed]

[4] Hall JL (2002). Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Botany; 53(366): 1-11. [CrossRef]

[5] Malekii M, Ghorbanpour M, Kariman K (2017). Physiological and antioxidative responses of medicinal plants exposed to heavy metals stress. Journal Plant Genetic; 11: 247–54. [CrossRef]

[6] Gjorgieva D, Kadifkova-Panovska T, Baceva K, Stafilov T (2011). Assessment of heavy metal pollution in Republic of Macedonia using a plant assay. Arch Environ Contam Toxicol; 60: 233-40. [CrossRef] [PubMed]

[7] Wierzbicka M, Obidzinska J (1998) The effect of lead on seed imbibition and germination in different plant species. Plant Science; 137: 155-71. [CrossRef]

[8] Yang Y, Liuqing Z, Xing H, Yiyang Z, Qiumei Q, Yunxiang L, Xiaohua Z (2020). Response of photosynthesis to different concentrations of heavy metals in Davidia involucrata. PLOS ONE; 15 (3): e0228563. [CrossRef] [PubMed]

[9] Woolhouse H.W. (1983). Toxicity and tolerance in the response of plants to metals. In: Lang OL, et al. (Eds.), Encyclopedia of Plant Physiology, Berlin: Springer-Verlag. p. 245-300.

[10] Kastori R, Maksimović I, Doroghazi O, Putnik-Delić M (2012), Effect of lead contamination of maize seed on its biological properties. Proc Nat Sci Matica Srpska - Novi Sad; 123: 75-82. [CrossRef]

[11] Nas FS, Ali M (2018). The effect of lead on plants in terms of growing and biochemical parameters: a review. Eco Environ Sci; 3(4): 265-268. [CrossRef]

[12] Chugh LK, Sawhney SK (1999). Photosynthetic activities of Pisum sativum seedlings grown in presence of cadmium. Plant Physiol Biochem; 37(4): 297–303. [CrossRef]

[13] Kastori R, Petrović N, Petrović M. (1996). Effects of lead on water relations, proline concentration and nitrate reductase activity in sunflower plants. Acta Agro Hung; 44(1): 21-8.

[14] de Varennes A, Torres MO, Coutinho JF, Rocha MMGS, Neto MMPM (1996). Effects of heavy metals on the growth and mineral composition of a nickel hyperaccumulator. J Plant Nutri; 19: 669–76. [CrossRef]

[15] Smith SR (1994). Effect of soil pH on availability to crops of metals in sewage sludge-treated soils. Nickel, copper and zinc uptake and toxicity to ryegrass., Environ Pollut; 85(3): 321-7. [CrossRef] [PubMed]

[16] Vasić T, Andjelković S, Radović J, Lugić Z, Hajnal-Jafari T, Djurić S, Živković S (2014). Alfalfa inoculation: the effect on root growth and number of rhizospheric microorganisms. Roman Biotechnol Lett; 19 (4): 9457-64.

[17] Sêkara A, Poniedziaek M, Ciura J, Jêdrszczyk E (2005). Cadmium and lead accumulation and distribution in the organs of nine crops: Implications for phytoremediation. Pol J Environ Stud; 14: 509-16.

[18] Chhotu JD, Fulekar MH (2008). Phytotoxicity and remediation of heavy metals by alfalfa (Medicago sativa) in soil-vermicompost media. Adv Nat Appl Sci; 2: 141–51.

[19] Gardea-Torresdey JL (2003). Phytoremediation where does it stand and where will it go? Environ Prog; 22; A2–A4. [CrossRef]

[20] Sethy SK, Ghosh S (2013). Effect of heavy metals on germination of seeds. J Nat Sci Biol Med; 4(2): 272–275. [CrossRef] [PubMed]

[21] Titov AF, Talanova VV, Boeva NP (1996). Growth responses of barley and wheat seedlings to lead and cadmium. Biologica Planatarum; 38(3): 431-436. [CrossRef]

[22] Kabir M, Zafar MZ, Shafiq M, Farooqi ZR (2008). Reduction In germination and seedling growth of Thespesia populnea L., caused by lead and cadmium treatments. Pak J Bot; 40: 2419-26.

[23] Tiemann KJ, Gardea-Torresdey JL, Gamez G, Dokken K, Sias S, Renner MW,Furenlid LR (1999). Use of X-ray Spectroscopy and Esterification to Investigate Cr(III) and Ni(II) Ligands in Alfalfa Biomass. Eviron Sci Technol; 33: 150-4. [CrossRef] [PubMed]

[24] Vasse J, de Billy F, Truchet G (1993). Abortion of infection during the Rhizobium meliloti—alfalfa symbiotic interaction is accompanied by a hypersensitive reaction. Plant J; 4(3): 555-566. [CrossRef]

[25] Sêdzik M, Smolik B, Krupa-Małkiewicz M (2015). Effect of lead on germination and some morphological and physiological parameters of 10-day-old seedlings of various plant species. Environment; 26: 22-7. [CrossRef]

[26] Peralta-Videa V, de la Rose G, Gonzales JH, Gardea-Torresdey J. (2004). Effects of the growth stage on the heavy metal tolerance of alfalfa plants. Adv Environ Res; 8(3-4): 679-85. [CrossRef]

[27] Arena C, Figlioli F, Sorrentino MC, Izzo LG, Capozzi, Giordano S, Spagnuolo V (2017). Ultrastructural, protein and photosynthetic alterations induced by Pb and Cd in Cynara cardunculus L., and its potential for phytoremediation. Ecotoxicol Environmen Safety; 145: 83-9. [CrossRef] [PubMed]

[28] Begonia MTF, Begonia A, Butler M, Ighoavodha B, Crudup B (2002). Chelate-assisted phytoextraction of lead from a contaminated soil using wheat (Triticum aestivum L.). Bull Environ Contam Toxicol; 68: 705–711. [CrossRef] [PubMed]



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